statistical parametric mapping software version 99 (spm 99 Search Results


microtubule associated proteins  (Cytoskeleton Inc)
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rep 2 atcc 824 capp  (ATCC)
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vwr extra pure  (Chem Impex International)
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canine reference genome canfam3.1  (Broad Institute Inc)
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anti map2  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology anti map2
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gene exp map2 hs00159041 m1  (Thermo Fisher)
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anti hsp70 antibody  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology anti hsp70 antibody
Figure 1. Characterization of uterine extracellular vesicles (uEVs). (A) Representative images (transmission electron microscopy) of EVs samples isolated from uterine lavages from pregnant mares on days 10 (P10), 11 (P11), 12 (P12), and 13 (P13), and from cyclic mares (controls) on days 10 (C10) and 13 (C13) after final ultracentrifugation and referred as uEVs. (B) Western blot characterization of uEVs (100,000 g) and the 12,000 g pellet for known exosomal protein markers CD9, TSG101, ALIX (PDCD6IP) and <t>HSP70.</t> (C) Comparison of uEVs concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300). (D) Comparison of uEVs protein concentration across samples measured by Pierce™ BCA protein assay. (E) Comparison of uEVs RNA concentration across samples measured by Quantus™ Fluorometer.
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optical maps  (BioNano Genomics)
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Figure 1. Characterization of uterine extracellular vesicles (uEVs). (A) Representative images (transmission electron microscopy) of EVs samples isolated from uterine lavages from pregnant mares on days 10 (P10), 11 (P11), 12 (P12), and 13 (P13), and from cyclic mares (controls) on days 10 (C10) and 13 (C13) after final ultracentrifugation and referred as uEVs. (B) Western blot characterization of uEVs (100,000 g) and the 12,000 g pellet for known exosomal protein markers CD9, TSG101, ALIX (PDCD6IP) and <t>HSP70.</t> (C) Comparison of uEVs concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300). (D) Comparison of uEVs protein concentration across samples measured by Pierce™ BCA protein assay. (E) Comparison of uEVs RNA concentration across samples measured by Quantus™ Fluorometer.
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2 7 dihydroxynapthalene  (Chem Impex International)
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Chem Impex International 2 7 dihydroxynapthalene
Figure 1. Characterization of uterine extracellular vesicles (uEVs). (A) Representative images (transmission electron microscopy) of EVs samples isolated from uterine lavages from pregnant mares on days 10 (P10), 11 (P11), 12 (P12), and 13 (P13), and from cyclic mares (controls) on days 10 (C10) and 13 (C13) after final ultracentrifugation and referred as uEVs. (B) Western blot characterization of uEVs (100,000 g) and the 12,000 g pellet for known exosomal protein markers CD9, TSG101, ALIX (PDCD6IP) and <t>HSP70.</t> (C) Comparison of uEVs concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300). (D) Comparison of uEVs protein concentration across samples measured by Pierce™ BCA protein assay. (E) Comparison of uEVs RNA concentration across samples measured by Quantus™ Fluorometer.
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bayesian technique  (DNAnexus Inc)
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DNAnexus Inc bayesian technique
Figure 1. Characterization of uterine extracellular vesicles (uEVs). (A) Representative images (transmission electron microscopy) of EVs samples isolated from uterine lavages from pregnant mares on days 10 (P10), 11 (P11), 12 (P12), and 13 (P13), and from cyclic mares (controls) on days 10 (C10) and 13 (C13) after final ultracentrifugation and referred as uEVs. (B) Western blot characterization of uEVs (100,000 g) and the 12,000 g pellet for known exosomal protein markers CD9, TSG101, ALIX (PDCD6IP) and <t>HSP70.</t> (C) Comparison of uEVs concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300). (D) Comparison of uEVs protein concentration across samples measured by Pierce™ BCA protein assay. (E) Comparison of uEVs RNA concentration across samples measured by Quantus™ Fluorometer.
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phospho p44 42 mapk  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc phospho p44 42 mapk
Figure 1. Characterization of uterine extracellular vesicles (uEVs). (A) Representative images (transmission electron microscopy) of EVs samples isolated from uterine lavages from pregnant mares on days 10 (P10), 11 (P11), 12 (P12), and 13 (P13), and from cyclic mares (controls) on days 10 (C10) and 13 (C13) after final ultracentrifugation and referred as uEVs. (B) Western blot characterization of uEVs (100,000 g) and the 12,000 g pellet for known exosomal protein markers CD9, TSG101, ALIX (PDCD6IP) and <t>HSP70.</t> (C) Comparison of uEVs concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300). (D) Comparison of uEVs protein concentration across samples measured by Pierce™ BCA protein assay. (E) Comparison of uEVs RNA concentration across samples measured by Quantus™ Fluorometer.
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high mapping similarity  (Laysan Bio)
86
Laysan Bio high mapping similarity
Figure 1. Characterization of uterine extracellular vesicles (uEVs). (A) Representative images (transmission electron microscopy) of EVs samples isolated from uterine lavages from pregnant mares on days 10 (P10), 11 (P11), 12 (P12), and 13 (P13), and from cyclic mares (controls) on days 10 (C10) and 13 (C13) after final ultracentrifugation and referred as uEVs. (B) Western blot characterization of uEVs (100,000 g) and the 12,000 g pellet for known exosomal protein markers CD9, TSG101, ALIX (PDCD6IP) and <t>HSP70.</t> (C) Comparison of uEVs concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300). (D) Comparison of uEVs protein concentration across samples measured by Pierce™ BCA protein assay. (E) Comparison of uEVs RNA concentration across samples measured by Quantus™ Fluorometer.
High Mapping Similarity, supplied by Laysan Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Characterization of uterine extracellular vesicles (uEVs). (A) Representative images (transmission electron microscopy) of EVs samples isolated from uterine lavages from pregnant mares on days 10 (P10), 11 (P11), 12 (P12), and 13 (P13), and from cyclic mares (controls) on days 10 (C10) and 13 (C13) after final ultracentrifugation and referred as uEVs. (B) Western blot characterization of uEVs (100,000 g) and the 12,000 g pellet for known exosomal protein markers CD9, TSG101, ALIX (PDCD6IP) and HSP70. (C) Comparison of uEVs concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300). (D) Comparison of uEVs protein concentration across samples measured by Pierce™ BCA protein assay. (E) Comparison of uEVs RNA concentration across samples measured by Quantus™ Fluorometer.

Journal: Scientific reports

Article Title: Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare.

doi: 10.1038/s41598-022-19958-z

Figure Lengend Snippet: Figure 1. Characterization of uterine extracellular vesicles (uEVs). (A) Representative images (transmission electron microscopy) of EVs samples isolated from uterine lavages from pregnant mares on days 10 (P10), 11 (P11), 12 (P12), and 13 (P13), and from cyclic mares (controls) on days 10 (C10) and 13 (C13) after final ultracentrifugation and referred as uEVs. (B) Western blot characterization of uEVs (100,000 g) and the 12,000 g pellet for known exosomal protein markers CD9, TSG101, ALIX (PDCD6IP) and HSP70. (C) Comparison of uEVs concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300). (D) Comparison of uEVs protein concentration across samples measured by Pierce™ BCA protein assay. (E) Comparison of uEVs RNA concentration across samples measured by Quantus™ Fluorometer.

Article Snippet: Antibodies and dilutions used for Western Blotting experiments were as follow: For primary antibodies, CD9 antibody (Mouse anti Human CD9, clone MM2/57, MCA469GT, specified as cross-reactive with equine CD9 by the provider Bio-Rad), dilution 1:500; TSG101 Polyclonal Antibody (PA5-31,260, rabbit, specificity demonstrated by shRNA mediated knockdown of the target protein, Invitrogen), 1:1000; Anti-ALIX antibody (1A12, mouse, monoclonal, sc-53540, Santa Cruz Biotechnology, Inc., Heidelberg, Germany, specificity shown in88), 1:500; Anti-HSP70 antibody (mouse, monoclonal, sc-66048, Santa Cruz, epitope mapping to amino acids 436–503 which are 99% identical between humans and horses, specificity shown in89), 1:500.

Techniques: Transmission Assay, Electron Microscopy, Isolation, Western Blot, Comparison, Concentration Assay, Protein Concentration, Bicinchoninic Acid Protein Assay